자외선 처리가 TiO2 나노튜브 위에 분주된 인간 중간엽 줄기세포의 부착, 성장 및 조골세포로의 분화에 미치는 효과
The Effect of the UV Treatment of TiO2 Nanotube Arrays on the Adhesion, Proliferation, and Osteogenic Differentiation of Human Mesenchymal Stem Cells
문경숙, 진수현, 배지명, 오승한,
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문경숙 ( Mon Kyoung-Sook ) - 원광대학교 치과대학 치과생체재료학교실
진수현 ( Jin Su-Hyun ) - 원광대학교 치과대학 치과생체재료학교실
배지명 ( Bae Ji-Myung ) - 원광대학교 치과대학 치과생체재료학교실
오승한 ( Oh Seung-Han ) - 원광대학교 치과대학 치과생체재료학교실
KMID : 0362220110380030189
Abstract
We prepared ultra violet (UV) light irradiated TiO2 nanotube array on eletropolished titanium surface and estimated the effect of the UV treatment of TiO2 nanotubular structure on the adhesion, proliferation, and osteogenic differentiation of human mesenchymal stem cells (hMSCs) in terms of different diameters (30 - 100 nm) of TiO2 nanotubes. Fluorescein diacetate assay resulted that the initial adhesion of hMSCs did not show any sign ifican t difference between all TiO2 nanotube diameter (p>0.05). However, the proliferation of hMSCs cultured on electropolished Ti surface were higher than those cultured on 50, 70, and 100 nm TiO2 nanotubes (p<0.05) From the results of RT-PCR assay, the values of i ntegrin β1, osteocalcin, osteoponting, and alkaline phosphatase did not show any significant difference between all experimental groups after 1 week of incubation period. However, the value of alkaline phosphatase showed the tendency of increasing
with proportional to the diameter of TiO2 nanotubes. Therefore, we can expect the UV treatment of TiO2 nanotube did not affect he adhesion, proliferation, and osteogenic differentiation of hMSCs compared to non UV treated TiO2 nanotube. Also, the enlargement of nanotube diameter is supposed to promote osteogenic differentiation of hMSCs from all results of this study.
키워드
Electropolishing;Human mesenchymal stem cells(hMSCs);osteogenic differentiation;TiO2 nanotube;UV treatment
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